In the course of a public appearance to Lichfield Cathedral on Monday, the King was heckled by a man who asked him about his brother's connections with convicted sex offender Jeffrey Epstein.
The individual asked how long the King had been aware about his sibling's association with Jeffrey Epstein, and also raised doubts about whether he had instructed the authorities to "cover up" for Prince Andrew.
Amid the heckling, there were crowds of fans and applause for the King, who was visiting Staffordshire for an ceremony.
The Duke of York's connections with Jeffrey Epstein have been under intense scrutiny in recent weeks. The royal, who gave up his titles earlier this month, has repeatedly denied any misconduct.
"For how long have you known about Prince Andrew and Epstein? Did you request the authorities to conceal for Andrew?"
The monarch ignored the questions but continued greeting supporters, some of whom chanted "Long live the King". One supporter can be overheard asking him for a embrace.
A female bystander outside the cathedral was also observed telling the protester to "go away" and to "shut up".
The controversy over the Duke of York's links to Epstein has been brought back into focus by misconduct claims repeated in Virginia Giuffre's posthumously-published memoir.
In the memoir, the accuser alleges she had sexual encounters with the prince on multiple times, including once with Epstein and "eight other young girls"
Andrew, who reached a out-of-court agreement with the plaintiff in recent years, has repeatedly denied any wrongdoing. In a previous interview, he told a news program he did not remember meeting her "at all", and that they "never had any sort of intimate relations."
But recently, fresh questions have been raised about how he is able to finance his way of life despite not being a working royal.
The disclosure that he has only ever paid a token annual rent on his Royal Lodge has intensified the scrutiny over his housing situation - even though he made large payments up front, including for renovations.
The political party said he should be called in front of members of parliament to give testimony about his rental agreement of his lodge.
But last Thursday, the government declined to give parliamentarians time to discuss the royal's titles or his home, despite increasing calls for investigation.
The royal household has not commented on whether the Duke of York might relocate of his home - or where he might reside.
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